Table A2.
Microsatellite loci and design of multiplex reactions: repeat motif, sequences of forward, F (fluorescently labeled) and reverse, R (pig-tailing: GTTTCTT added at 5′-end) primer, fluorescent dye, primer final concentration, and the original reference describing the primers are given for each locus
| Mix | Locus | Repeat motif | Primer sequences (5'-3') | Dye | Concentration (μM) | Reference |
|---|---|---|---|---|---|---|
| Caud13 | (AC)n | F: ACAATAGATTCCAGATGCTGAA | PET | 0.5 | Huang et al. 2006 | |
| R: ATGTCTGAGTCCTCGGAGC | ||||||
| MM07 | (CA)n | F: GCAAAAGGGGCTATGAACAC | NED | 1.25 | Hefti-Gautschi & Koller 2005 | |
| R: GGAGGCAGAGCTGGTTAGTG | ||||||
| Sfiμ3 | (GA)nN2(GA)n | F: TATTCCAATATTCTGCAGGGAGG | 6-FAM | 1.25 | Fields and Scribner 1997 | |
| R: TCCAAGTTAATCAATTATCTGAT | ||||||
| SET1 | Smo11 | (TG)nGA | F: AAATCAACCAAAGAGGCATAGCC | 6-FAM | 2.0 | Paulus and Tiedemann 2003 |
| R: GCAGTTGTTTTGGAGGACAGACA | ||||||
| Sfiμ4 | (GA)n | F: CTGAGGGGGAAGAGAATAAGAGA | PET | 3.0 | Fields and Scribner 1997 | |
| R: CAGGGCAGTATTTTCAGGACATT | ||||||
| MM05 | (AC)n | F: CCAAATCTGACCACCAGGAG | VIC | 3.0 | Hefti-Gautschi and Koller 2005 | |
| R: GCCGTCAGGCAAATAGGAAC | ||||||
| CmAAT28 | (AAT)n | F: TGAAAAGGGTCTTTACCTTATAT | 6-FAM | 2.0 | Stai and Hughes 2003 | |
| R: TTCCACATAAAAATTCATTCAGT | ||||||
| Apl12 | (GA)n | F: AGTTGACCCTAATGTCAGCATC | 6-FAM | 1.25 | Denk et al. 2004 | |
| R: AAGAGACACTGAGAAGTGCTATTG | ||||||
| CmAAT38 | (AAT)n | F: TCCCGAGCTACCACATTGAC | NED | 3.0 | Stai and Hughes 2003 | |
| R: AGCAACTGGAAGGCATTTATCT | ||||||
| Smo4 | (AG)nA | F: ACTTTCCACAGCCTCTTTCACAA | VIC | 2.5 | Paulus and Tiedemann 2003 | |
| R: GACAGTGTTTGTCAATGGATTTT | ||||||
| SET2 | Aph13 | (GA)n | F: CAACGAGTGACAATGATAAAA | 6-FAM | 1.25 | Maak et al. 2003 |
| R: CAATGATCTCACTCCCAATAG | ||||||
| MM03 | (CA)n | F: AAGTACATGTAAAAGCTGAAGTTGC | PET | 3.0 | Hefti-Gautschi and Koller 2005 | |
| R: TTGCCTGATAAAAGGAATGC | ||||||
| Apl36 | (CA)n | F: ATGCTTTGCTGTTGGAGAGC | NED | 3.0 | Denk et al. 2004 | |
| R: TCCACTGGGTGCAAACAAG | ||||||
| Sfiμ2 | (A)n(CA)n | F: CATAAACGGCTAATATGAAGTCT | 6-FAM | 2.0 | Fields and Scribner 1997 | |
| R: AGGCTAGATATTGCTCTTATCCT |