Fig. 7.

Insulin evokes mobilization of an NADPH oxidase in podocytes. A: cell surface biotinylation assay showing that insulin treatment for 24 h markedly increased surface expression of the higher molecular weight form of NOX4 in podocytes. B: summary of densitometric quantification of 3 repetitions of the experiment shown in A. C: immunoblot analysis showing effective knockdown of total NOX4 expression 24 h after transient transfection with an appropriate siRNA. Control cells were transfected with a nontargeted siRNA. D: summary of 3 repetitions of the experiment shown in C.