Micro-CT. A: representative 3-dimensional (3D) micro-CT reconstruction of the renal vasculature. B: quantification of the cortical microvascular (MV) density and vascular volume fraction of microvessels with diameters between 0–100, 200–300, and 300–500 μm. Data are expressed as means ± SE. 4w, 4 wk; 12w, 12 wk; ND, nondiabetic; D, diabetic.