Fig. 11.

A representative recording from a VP neuron showing the effect of benzamil on the current measured at −70 mV. The steady-state current was measured when the cell was held at −70 mV in voltage clamp. Brief hyperpolarizing pulses (200 ms, −15 mV) were injected every 5 s to monitor the input resistance of the cell. Washing amiloride from the bath resulted in an increase in a resting inward current and a decreased resistance. Subsequent bath application of ENaC blocker benzamil (1 μM) reduced the resting inward current and decreased conductance ∼1.5-fold. The effect was reversed by wash of benzamil by artificial cerebrospinal fluid (ACSF).