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. 2011 Nov 1;302(3):E273–E285. doi: 10.1152/ajpendo.00407.2011

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Fig. 2. — Colocalization of α-epithelial Na⁺ channel (ENaC) subunit with both VP-NP and OT-NP immunoreactivity in coronal section of the rat supraoptic nucleus (SON). A and D: α-ENaC subunit immunoreactivity labeled with DyLight 488-conjugated secondary antibody. Note that the intense immunoreactivity to α-ENaC subunit appeared to be confined in somata of the MNCs within the SON. B: VP-NP immunoreactivity labeled with DyLight 594-conjugated secondary antibody in same section and image plane as in A. E: OT-NP immunoreactivity labeled with DyLight 594-conjugated secondary antibody in same section and image plane as in D. C and F: the merged images revealed that α-ENaC immunoreactivity is colocalized with both VP and OT immunoreactivities within MNCs in the SON.