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. 2011 Nov 1;302(3):E273–E285. doi: 10.1152/ajpendo.00407.2011

Fig. 5.

Fig. 5.

Colocalization of β-ENaC subunit and VP-NP immunoreactivity in coronal section of the rat PVN. A and D: β-ENaC subunit immunoreactivity labeled with DyLight 488-conjugated secondary antibody. Note that intense immunoreactivity to β-ENaC is observed in rather scarcely distributed MNCs located mostly within the lateral portion of the PVN. However, unlike in the SON, the immunoreactivity in these cells is not confined to the soma. Prominent immunoreactivity can be seen in the dendritic processes of these neurons. There is no prominent immunoreactivity to β-ENaC observed in the parvocellular cells. B: VP-NP immunoreactivity labeled with DyLight 594-conjugated secondary antibody in same section and image plane as in A. Note that the VP-NP immunoreactive cells form a cluster in the lateral portion of the PVN. E: OT-NP immunoreactivity labeled with DyLight 594-conjugated secondary antibody in same section and image plane as in D. C: the merged images revealed that β-ENaC immunoreactivties appear to be colocalized only with VP within the MNCs (arrows). F: the merged image of D and E. Note that β-ENaC immunoreactivty is not colocalized with OT (arrows).