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. 2011 Nov 1;302(3):E273–E285. doi: 10.1152/ajpendo.00407.2011

Table 1.

Primer sequences used to detect gene expressions of interest

Gene Primer Sequence Amplicon Size, bp
α-ENaC
    Forward 5′-GTTCTGTGACTACCGAAAGCAGAG-3′ 429
    Reverse 5′-CGTAGCAGCATGAGAAGTGTGATG-3
β-ENaC
    Forward 5′-ACCCTGAGCAGGAAGGGTAT-3′ 220
    Reverse 5′-ACAGGAGGCCACTAGCTTGA-3
γ-ENaC
    Forward 5′-CGTCAGTGGCACAAAGCCAA-3′ 301
    Reverse 5′-GAGAGCCTCCTCAAACCATG-3′
MR
    Forward 5′-GCTCAACATTGTCCAGTACA-3′ 260
    Reverse 5′-GCACAGGTGGTCCTAAGATT-3′
OT
    Forward 5′-GACGGTGGATCTCGGACTGAA-3′ 463
    Reverse 5′-CGCCCCTAAAGGTATCATCACAAA-3′
VP
    Forward 5′-CCTCACCTCTGCCTGCTACTT-3′ 440
    Reverse 5′-GGGGGCGATGGCTCAGTAGAC-3′

ENaC, epithelial Na+ channel; MR, mineralocorticoid receptor; OT, oxytocin; VP, vasopressin.