Table 1.
Gene | Primer Sequence | Amplicon Size, bp |
---|---|---|
α-ENaC | ||
Forward | 5′-GTTCTGTGACTACCGAAAGCAGAG-3′ | 429 |
Reverse | 5′-CGTAGCAGCATGAGAAGTGTGATG-3 | |
β-ENaC | ||
Forward | 5′-ACCCTGAGCAGGAAGGGTAT-3′ | 220 |
Reverse | 5′-ACAGGAGGCCACTAGCTTGA-3 | |
γ-ENaC | ||
Forward | 5′-CGTCAGTGGCACAAAGCCAA-3′ | 301 |
Reverse | 5′-GAGAGCCTCCTCAAACCATG-3′ | |
MR | ||
Forward | 5′-GCTCAACATTGTCCAGTACA-3′ | 260 |
Reverse | 5′-GCACAGGTGGTCCTAAGATT-3′ | |
OT | ||
Forward | 5′-GACGGTGGATCTCGGACTGAA-3′ | 463 |
Reverse | 5′-CGCCCCTAAAGGTATCATCACAAA-3′ | |
VP | ||
Forward | 5′-CCTCACCTCTGCCTGCTACTT-3′ | 440 |
Reverse | 5′-GGGGGCGATGGCTCAGTAGAC-3′ |
ENaC, epithelial Na+ channel; MR, mineralocorticoid receptor; OT, oxytocin; VP, vasopressin.