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. 2012 Feb 6;122(3):859–872. doi: 10.1172/JCI60818

Figure 3. DYRK2 regulates c-Jun and c-Myc stability.

Figure 3

(A) U2OS cells were transfected with scrambled siRNA or DYRK2 siRNA. After transfection, cells were incubated with 40 μg/ml CHX for the indicated times. Lysates were analyzed by immunoblotting with anti–c-Jun, anti–c-Myc, or anti-tubulin. The signals for c-Jun (left) or c-Myc (right) were scanned to compare the amount in control (time 0). *P < 0.05. Data represent mean ± SD. (B) U2OS cells were transfected with scrambled siRNA or DYRK2 siRNA and incubated with 10 μM MG-132 for 4 hours. Lysates were immunoprecipitated with rabbit IgG, anti–c-Jun, or anti–c-Myc followed by immunoblotting with anti-ubiquitin, anti–c-Jun, or anti–c-Myc. Lysates were also analyzed by immunoblotting with indicated antibodies (right panels). (C) U2OS cells were transfected with scrambled siRNA, DYRK2 siRNA, or Fbw7 siRNA. Lysates were immunoblotted with anti–c-Jun, anti–c-Myc, or anti-tubulin. The lanes separated by the white line were run on the same gel, but were noncontiguous. Total RNAs were analyzed by RT-PCR using DYRK2-specific, Fbw7-specific, or actin-specific primers.