Fig. 1.

Intralipid treatment induces steatosis and uncoupling protein 2 (UCP2) upregulation in hepatocytes in vitro. Wild-type (WT) and UCP2 knockout (KO) mouse hepatocytes were cultured in the absence (control) or presence of 6% intralipid (emulsified fatty acids) for 24 h. A: cells were stained with oil red O (ORO) to visually assess the development of the steatotic phenotype and were counterstained with hematoxylin. Photomicrographs were captured at ×200. B: mitochondrial isolates were probed for UCP2, and cytochrome c oxidase subunit IV was detected to confirm equivalent loading. Mean values standardized to cyclooxygenase (COX) IV + SD are represented. n = 3/group, *P < 0.05.