Table 1.

Fatty acid concentrations in fresh and cultured (24 h control vs. intralipid) WT and UCP2 KO hepatocytes

	Fresh		Control Media		Intralipid Media
Fatty Acids	WT	KO	WT	KO	WT	KO
Arachidonic	5343 ± 1273	4507 ± 2015	5569 ± 2258	5230 ± 1759	11716 ± 1190	11025 ± 1848
Caprylic	196 ± 139	179 ± 91	327 ± 145	260 ± 84	840 ± 145	1010 ± 214
Cis-Docosahexaenoic	3153 ± 706	2765 ± 1607	3065 ± 1613	3097 ± 1638	7866 ± 1425	9811 ± 1633
Cis-Eicosatrienoic	208 ± 288	272 ± 241	739 ± 298	449 ± 257	0 ± 0	0 ± 0
α Linolenic	0 ± 0	0 ± 0	0 ± 0	80 ± 180	2584 ± 711	1982 ± 1094
Linoleic	8337 ± 1672	7743 ± 2684	9336 ± 2801	8988 ± 2108	39065 ± 7387	41572 ± 8086
Oleic	4383 ± 1347	4340 ± 1314	5036 ± 1882	5042 ± 1398	22681 ± 3667	27839 ± 2646
Palmitic	9485 ± 1955	8494 ± 3104	11303 ± 3271	9432 ± 2173	22918 ± 2793	25091 ± 3029
Palmitoleic	537 ± 292	364 ± 158	490 ± 293	529 ± 242	2629 ± 1279	2974 ± 1187
Stearic	5599 ± 821	4227 ± 1849	6286 ± 1882	5773 ± 1750	11162 ± 1685	13107 ± 1994

Data are means ± SD and represent ng/mg protein. For all fatty acids except cis-eicosatrienoic, concentrations are greater in the intralipid treated vs. freshly isolated and control cultured hepatocytes, P < 0.05. Wild-type (WT) vs. knockout (KO) intralipid for all groups, no difference. n = 5/group. UCP2, uncoupling protein-2.