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. 2012 Feb 10;2(2):179–189. doi: 10.7150/thno.3716

Figure 6.

Figure 6

NIRF signal of cathepsin B and MMP probes in excised SCC7 tumors and other visceral organs. (A) Representative ex vivo NIRF imaging of cathepsin B probe and MMPs injected tumor bearing mice scarified at fluorescence intensity peak time. (B) Comparison of the NIRF intensity of two probes in visceral organs and tumor. (C) Cathepsin B expression in the cytoplasm of the tumor cells (brown). Immunohistochemistry (IHC); horse radish peroxidase and diaminobenzidine (HRP-DAB)/hematoxylin. (D) NIRF of cathepsin B probe in tumor tissue (red). Fluorescence imaging; 695 nm. (E) Cathepsin B negative hepatocytes and cathepsin B stained vascular endothelia in liver. (F) NIRF of cathepsin B probe in liver tissues. (G) Diffusely stained tumor cells with MMP-13 antibody. (H) NIRF of MMP probe in tumor tissue (I) MMP-13 negative hepatocytes. (J) Low NIRF recovery of MMP probe in liver tissues.