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(a) Schematic structures of cDNA constructs. Fluc-N and Fluc-C indicate N-terminal and C-terminal fragments of Fluc. The Flanking sides of the luciferase are connected with C-terminal and N-terminal fragments of DnaE (DnaEc and DnaEn). A PEST sequence is attached at the C-terminal end to decompose the unspliced product. (b) Principle for monitoring activity of caspase-3 using cyclic firefly luciferase.
