Table 4.
Expression of the candidate genes involved in the A. vulgare immune response. Transcripts of genes were quantified by RT-qPCR and normalized with the expression of the L8 ribosomal protein (RbL8) and the Elongation Factor 2 (EF2). The ratio of expression between symbiotic and asymbiotic conditions was calculated for each sample (F=whole females; Ov=ovaries; IT=immune tissues, see text). Over-expression and under-expression in symbiotic samples were highlighted in light grey and in dark grey respectively (* p<0.05; ** p<0.001; - no measurable response).
| ratio symbiotic /asymbiotic | |||||
|---|---|---|---|---|---|
| Biological functions | Genes | F | Ov | IT | |
| Pathogen Detection | Recognition | C-type lectin 1 | 1.19 | 3.42** | 1.55 |
| C-type lectin 2 | 0.90 | 0.30** | - | ||
| C-type lectin 3 | 0.47* | - | 1.06 | ||
| Peroxinectin-like A | 0.93 | 0.09 | 2.03 | ||
| Peroxinectin-like B | 0.72 | 0.93 | 2.03 | ||
| Transduction | ECSIT | 1.44 | 0.63 | 1.48 | |
| MyD88-like | 0.86 | 0.78 | 1.45 | ||
| SOCS2-like | - | 0.72 | 1.44 | ||
| Immune response | AMP | ALF 1 | 0.77 | 0.57 | 0.68 |
| ALF 2 | 0.90 | 2.50 | 1.42 | ||
| Armadillidine | 0.44** | 0.83 | 0.95 | ||
| Crustin 1 | 0.57 | - | - | ||
| Crustin 2 | 0.77 | 0.48 | - | ||
| Crustin 3 | 0.50** | 0.47** | - | ||
| i-type lyzozyme | 0.63** | 0.44 | 1.77 | ||
| Serine proteases | Masquerade-like A | 0.41 | 1.30 | 1.18 | |
| Masquerade-like B | 0.36* | 0.33 | - | ||
| Serine protease inhibitors | α2-macroglobulin A | 0.95 | 1.03 | 1.05 | |
| α2-macroglobulin B | 0.80 | 0.83 | 1.21 | ||
| α2-macroglobulin C | 0.68 | 0.32** | 0.74 | ||
| α2-macroglobulin D | 0.56 | 1.88 | 1.47 | ||
| α2-macroglobulin E | 1.44 | 1.68 | 3.05 | ||
| Regulation of granular secretion | Cyclophilin G | 0.94 | 0.74 | 1.31 | |
| RNAi | Piwi | 0.95 | 0.74 | - | |
| Argonaute-like | 0.98 | 0.62 | 1.31 | ||
| Stress response/Detoxification | Ferritin A | 0.95 | 2.32* | 1.71 | |
| Ferritin B | 0.79 | 0.67 | - | ||
| Ferritin C | 0.84 | 1.90** | 1.65 | ||
| BIP2 | 0.86 | 0.57 | 1.23 | ||
| Peroxiredoxin A | 0.45 | 0.39 | 1.59 | ||
| Peroxiredoxin B | 0.58 | 0.44** | 1.05 | ||
| Peroxiredoxin C | - | 0.02** | - | ||
| Peroxiredoxin-like D | 0.71 | 1.16 | 0.53 | ||
| Thioredoxin A | 1.59 | 1.91** | 2.13 | ||
| Thioredoxin B | 0.57 | 1.17 | 0.73 | ||
| Glutathione peroxidase | 0.82 | 0.17** | 1.09 | ||
| Cu/Zn SOD | 0.45 | 0.68 | 1.12 | ||
| cytMn SOD | 0.65 | 0.77 | 1.66 | ||
| Coagulation | Transglutaminase A | 0.75 | 2.67 | 1.95 | |
| Transglutaminase B | 1.33 | 1.99 | 1.77 | ||
| Cellular differenciation | Astakine | 0.98 | 0.49 | 2.08 | |
| Runt | 1.40 | 0.83 | 1.69 | ||
| Apoptosis | AIF-like | - | 0.59 | - | |
| Autophagy | atg7 | 0.73 | 0.53** | 0.59 | |
| atg12 | 0.92 | 0.27* | 0.69 | ||
| Other | Cytoskeleton | Kinesin | 0.94 | 0.34 | 1.35 |
| S >A | S < A | ||||