Table 2.
Relative abundance of Gfp-tagged Asaia and Asaia sp. within the bacterial community of samples.
| GfpABR | ABR | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Sample and transmission type | Average (SD) | 24h | 48h | 72h | 96h | Average (SD) | 24h | 48h | 72h | 96h |
| Insect – Donors | 0.00724 (0.03573) | - | - | - | - | 0.05783 | - | - | - | - |
| Insect –Co-feeding | 0.00145 (0.00166) | 0.0000004 | 0.00212 | 0.00349 | 0.00019 | 0.04239 (0.04745) | 0.00002 | 0.08202 | 0.08490 | 0.00263 |
| Insect –Venereal transfer, ♂ to ♀ | 0.00105 (0.00179) | 0.0000003 | 0.00372 | 0.00004 | 0.00043 | 0.02277 (0.02602) | 0.05436 | 0.03381 | 0.00032 | 0.00258 |
| Insect –Venereal transfer, ♀ to ♂ | 0.00137 (0.00025) | - | 0.00119 | - | 0.00155 | 0.04265 (0.05056) | - | 0.07840 | - | 0.00690 |
| Diet –Co-feeding | 0.06143 (0.04979) | 0.12291 | 0.02367 | 0.08079 | 0.01833 | 0.35694 (0.40712) | 0.95646 | 0.09473 | 0.26633 | 0.11026 |
| Diet –Venereal transfer, ♂ to ♀ | 0.00070 (0.00045) | 0.00038 | 0.00102 | 0.09653 (0.13157) | - | - | 0.18957 | 0.00350 | ||
| Diet –Venereal transfer, ♀ to ♂ | 0.00490 (0.00501) | - | 0.00135 | - | 0.00844 | 0.02983 (0.00491) | - | 0.03330 | - | 0.02636 |
GfpABR (Gfp-tagged Asaia to Bacteria ratio) calculated as the ratio between the gfp copy number and the 16S rRNA gene copy number of the total bacterial community of the samples. ABR (Asaia to Bacteria ratio) calculated as the ratio between the number of Asaia cells and the total bacteria 16S rRNA gene copy number. In case of insect samples, all of the final copy numbers were calculated per pg of insect 18Sr RNA gene. Values in the Average column represent the average results of each group of trials for insect and diet samples; standard deviation is indicated in parenthesis.