Table 3.
Experimental design of S. titanus transmission trials.
| No. of individuals (donors + receivers) | Transmission type | Acquisition time | Destination |
|---|---|---|---|
| 20 (10 + 10) | Co-feeding with Asaia | 24 hours | q-PCR |
| 38 (19 + 19) | 48 hours | ||
| 28 (14 + 14) | 72 hours | ||
| 20 (10 + 10) | 96 hours | ||
| 8 (4 + 4) | 48 hours | FISH | |
| Tot. co-feeders: 114 (57 + 57) | |||
| 10 (5 + 5) | Asaia Venereal transfer (male to female) | 24 hours | q-PCR |
| 10 (5 + 5) | 48 hours | ||
| 10 (5 + 5) | 72 hours | ||
| 14 (7 + 7) | 96 hours | ||
| 10 (5 + 5) | 48 hours | FISH | |
| 10 (5 + 5) | Asaia Venereal transfer (female to male) | 24 hours | q-PCR |
| 14 (7 + 7) | 48 hours | ||
| 10 (5 + 5) | 72 hours | ||
| 12 (6 + 6) | 96 hours | ||
| 8 (4 + 4) | 48 hours | FISH | |
| Tot. mated: 108 (54 + 54) | |||
| 6 (3 + 3) | Co-housing control trial (males with males) | 24 hours | |
| 6 (3 + 3) | 48 hours | ||
| 6 (3 + 3) | 72 hours | ||
| 6 (3 + 3) | 96 hours | ||
| 10 (5 + 5) | Co-housing control trial (females with females) | 24 hours | q-PCR |
| 6 (3 + 3) | 48 hours | ||
| 6 (3 + 3) | 72 hours | ||
| 6 (3 + 3) | 96 hours | ||
| Tot. co-housed: 52 (26 + 26) | |||
| 20 (10 + 10) | Negative control for Co-feeding | 24 hours | q-PCR |
| 22 (11 + 11) | 48 hours | ||
| 28 (14 + 14) | 72 hours | ||
| 32 (16 + 16) | 96 hours | ||
| 10 (5 + 5) | 48 hours | FISH | |
| Tot. co-feeders: 112 (56 + 56) | |||
| 16 (8 + 8) | Negative control for venereal transfer (male to female) | 24 hours | q-PCR |
| 10 (5 + 5) | 48 hours | ||
| 8 (4 + 4) | 72 hours | ||
| 14 (7 + 7) | 96 hours | ||
| 10 (5 + 5) | 48 hours | FISH | |
| 8 (4 + 4) | Negative control for venereal transfer (female to male) | 24 hours | q-PCR |
| 14 (7 + 7) | 48 hours | ||
| 12 (6 + 6) | 72 hours | ||
| 10 (5 + 5) | 96 hours | ||
| 10 (5 + 5) | 48 hours | FISH | |
| Tot. mated: 112 (56 + 56) | |||
Number of insect specimens used for each trial. The duration of the acquisition period, as well as the type of analysis carried out, are indicated both for samples submitted to experiments performed with Gfp-tagged Asaia and for negative controls.