Figure 3.

Elevated cAMP and skewed PDE isoform expression in neonatal macrophages. AMs were harvested from adult ((~10 weeks old) and young (day 7 and 14) rats and allowed to adhere overnight. A. AMs were stimulated with media or 1 μM PGE₂ for 20 min before lysing the cells. Intracellular cAMP levels were measured in the whole cell lysate by specific EIA. For cAMP assay, data shown are representative of three individual experiments at least in preformed in duplicate. B. RNA was extracted from the cells, converted to cDNA and the relative expression of each of the PDE3 and PDE4 isoforms was determined using semi-quantitative real-time PCR. For real time PCR experiments, data represents mean ± SEM of pooled samples from 3 independent experiments. (*p < 0.05 and ***p < 0.001 when compared to the unstimulated samples (cAMP assay) and compared to adult AMs (real time PCR)).