Figure 4.

No difference in eicosanoid receptor expression in young AMs compared to adult AMs. AMs were harvested from adult ((~10 weeks old) and young (day 7 and 14) rats. RNA was extracted from the cells, converted into cDNA and the relative expression was determined using semi-quantitative real-time PCR. The primers for the specific genes of interest are located in Table 1. For each sample, the expression of the gene of interest was normalized to β-actin and the average of the adult AMs was set to 1. Data represents the mean ± SEM of pooled samples from 3 independent experiments.