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. 2012 Feb 27;7(2):e29527. doi: 10.1371/journal.pone.0029527

Figure 2. Highly conserved regulatory regions, upstream of the NAGS gene, function as promoter and enhancer elements.

Figure 2

In liver derived cells the NAGS promoter (4.10Prom), promoter+enhancer (4.10PromEnh), enhancer with TATA promoter (4.23Enh), and positive control promoter vector (pGL4.13) significantly simulate transcription while the enhancer (4.10Enh), basic vector (pGL4.10) does not stimulate transcription above baseline (A). Reverse insertion of the promoter (4.10PromRev) did not stimulate transcription compared to 4.10Prom and pGL4.10 vector (B), but reverse enhancer (4.23EnhRev) significantly stimulated transcription compared to 4.23Enh and pGL4.23 vector (C). Calculated results are an average of three independent experiments that were each carried out in triplicate, normalized to Rluc expression, and expressed relative to the promoter for each experiment with error reported as ±SEM. Lowercase letters indicate statistically significant differences.