Figure 4. Sequence alignment of NAGS promoters and enhancers from seven mammalian species indicate conserved motifs.

DNA sequence of the promoter (A) and enhancer (B) regions were aligned using CLUSTALW alignment software. CLOVER analysis was used to identify transcription factor binding motifs. Binding sites for C/EBP (green), Sp1 (red), CREB/ATF (pink), AP-2 (purple), HNF-1 (blue), NF-Y (olive), and SMAD 3 (cyan) were highly conserved.