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. 2012 Feb 27;7(2):e29527. doi: 10.1371/journal.pone.0029527

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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ChIP with transcription factor antibodies was compared to negative control IgG antibody. Real-Time PCR using promoter or enhancer specific primers was compared to primers for the negative locus MIP-2. The results confirmed that Sp1 and CREB but not C/EBP bind within the promoter (A) and HNF-1 and NF-Y but not AP-2 or SMAD2/3 bind within the enhancer region (B) of NAGS. Calculated error was from three replicate experiments and reported as ± SEM. One asterisk (*) indicates p<0.05 and two asterisks (**) indicate p<0.005.