Figure 7. Transcription factors Sp1, CREB, HNF-1, and NF-Y are functionally important for stimulating expression of reporter gene transcription.

Mutagenesis of the putative transcription factor binding sites significantly decreases transcription by the promoter (A) and the enhancer with TATA promoter (B) in liver derived cells when compared to non-mutated sites. Addition of Sp1 with the promoter (A) and HNF-1 with the enhancer (B) increases transcription driven by non-mutated constructs. Calculated results are an average of three independent experiments that were each carried out in triplicate, normalized to Rluc expression, and expressed relative to the promoter for each experiment with error reported as ±SEM. Lowercase letters indicate statistically significant differences.