Figure 5.

Atg16L2 is unable to localize on phagophores even under starvation conditions. (A–D) EGFP-Atg16L2 was transiently expressed in MEF cells stably expressing HA-ULK1 and in wild-type MEF cells. The starved cells were fixed and stained with anti-HA antibody (A), anti-Atg12 antibody (B), and anti-Atg16L1 antibody (C and D). Arrows indicate phagophores. A magnified view of the boxed area in (C) is shown in (D). Note that EGFP-Atg16L2 did not form any dots and that it did not colocalize with any of three phagophore markers, ULK1, Atg12 and Atg16L1. Scale bars, 20 μm.