Skip to main content
. 2011 Dec 1;7(12):1528–1545. doi: 10.4161/auto.7.12.18051

Figure 5.

Figure 5.

Autophagy flux measurements suggest enhancement of macroautophagy. (A) Western blotting of LC3 in cells cultured with or without lysosomal enzyme inhibitors E64d (10 μM) and Pepstatin A (Pep.A; 10 μg/ml) in normoxic conditions. (B) Increased LC3-II levels (normalized to actin) after lysosomal enzyme inhibition (*p < 0.05; n = 4). (C) Western blotting of LC3 in cells cultured with or without lysosomal enzyme inhibitors E64d (10 μM) and Pepstatin A (10 μg/ml) in hyperoxic conditions [same groups as in (A)]. (D) Increased LC3-II levels (normalized to actin) after lysosomal enzyme inhibition (*p < 0.05; n = 4). The elevated levels of LC3-II after lysosomal enzyme inhibition indicate an increased autophagic flux.