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. 2011 Dec 1;7(12):1528–1545. doi: 10.4161/auto.7.12.18051

Figure 9.

Figure 9.

Downregulation of ATG5 by siRNA protected cells from apoptosis and decreased the intralysosomal accumulation of APP and Aβ. (A) Western blot of ATG5 in APPswe cells (control), cells with scramble RNA transfection (scRNA) or ATG5 siRNA transfection. (B) Single immunostaining of LC3 (green fluorescence) in APPswe cells (control), cells with scRNA, and with ATG5 siRNA after 3 d exposure to hyperoxia. Both control and scRNA cells showed brighter and more granular staining of LC3 compared with ATG5 siRNA cells. Bar, 40 μm. (n = 3). Nuclei were stained by DAPI (blue fluorescence). (C–F) Vector and APPswe cells (controls), cells with scRNA, or ATG5 siRNA were exposed to normoxia and hyperoxia for 3 d. The number of cells with: nuclear fragmentation (C) and with APP (D), Aβ1–42 (E), or Aβ oligomer (F) -positive lysosomes were counted. ATG5 siRNA protected cells from death (C), and decreased lysosomal accumulation of APP (D), Aβ1–42 (E), and Aβ oligomers (F) (*p < 0.05; n = 3).