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. 2012 Feb 27;7(2):e31811. doi: 10.1371/journal.pone.0031811

Figure 4. (+)-Rutamarin selectively activates RXRα:RXRα and PPARγ:RXRα transcriptional activities.

Figure 4

HEK293 cells are transiently transfected by Lipo2000 respectively with RXRα, PPARγ, FXR, and LXRα plasmids plus pGL3-RXRE luciferase vector and renilla luciferase vector for 5 hours. Cells are then incubated with indicated concentrations of compounds for another 24 h and finally lysed. Luciferase activities are measured using Dual Luciferase Assay System kit. Rut dose-dependently activates (a) RXRα:RXRα and (b) PPARγ:RXRα activities. Rut has no effects on (c) FXR: RXRα and (d) LXRα:RXRα activities. Data are presented as means ± s.e.m (*P<0.05, **P<0.01) from three independent experiments.