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. 2012 Feb 27;7(2):e31811. doi: 10.1371/journal.pone.0031811

Figure 6. (+)-Rutamarin increases GLUT4 promoter activity through RXRα.

Figure 6

(a) Knockdown plasmid pSuper-RXRα or pSuper-PPARγ is transiently transfected into HEK293 cells by Lipo2000 for 5 hours, and the knockdown efficiency is determined by western bloting with antibody against RXRα or PPARγ. (b) In the presence of rxrα siRNA, Rut (20 µM) loses its ability to activate GLUT4 promoter, while similar activation of the promoter is found when pSuperbasic control vector is co-transfected. (c) pparγ siRNA has no effects on the activation of GLUT4 promoter by Rut (20 µM). Data are presented as means ± s.e.m (*P<0.05,**P<0.01) from three independent experiments.