Table 1. Species lineages.

Species	Lineage	Population	Location	Year of Collection
D. persimilis	111.01	-	Quesnel, British Columbia	1958
	111.46	-	Mount St. Helena, California	1997
	111.48	-	Mount St. Helena, California	1997
	111.5	-	Santa Cruz Island, California	2004
	111.51	-	Santa Cruz Island, California	2004
D. pseudoobscura	121.88	allopatric	Edo de Mexico, Mexico	1958
	121.89	allopatric	Edo de Mexico, Mexico	1958
	121.94	allopatric	Mesa Verde, Colorado	1996
	121.151	allopatric	Flagstaff, Arizona	1993
	121.15	allopatric	Organ Pipe Monument, Arizona	2007
	121.153	allopatric	Wilcox, Arizona	2007
	121.41	sympatric	Mather, California	1959
	121.42	sympatric	Mather, California	1959
	121.64	sympatric	Mather, California	1959
	Mather 17	sympatric	Mather, California	1997
	Mather 32	sympatric	Mather, California	1997
	121.103	sympatric	Mather, California	2001
	121.104	sympatric	Mather, California	2001
	121.148	sympatric	San Francisco, California	2006
	121.155	sympatric	Eugene, Oregon	2006

Fly lineages used in outcrossing and subsequent courtship trials. Lineages grouped in boxes were crossed to generate an outcrossed population from which flies were collected for experiment. 111.01 was not outcrossed as no additional population from that collection year was available. All lines were from the Drosophila Species Stock Center, except Mather 17 and 32 (provided by M. Noor).