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. 2012 Feb 27;7(2):e32039. doi: 10.1371/journal.pone.0032039

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Idris Khodja et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The expression level of eNOS, SK_Ca and IK_Ca (small- and intermediate-conductance Ca²⁺-activated K+ channel, respectively) was determined in mesenteric artery sections using purified polyclonal antibodies and a fluorescence-tagged secondary antibody by confocal microscopy. Left panels show representative immunofluorescence staining and right panels corresponding cumulative data. Results are shown as mean ± SEM of 4 different rats. *P<0.05 indicates a significant difference versus the young rat control group and ^# P<0.05 versus the middle-aged rat control group. All micrographs were taken at the same magnification.