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. 2012 Feb 27;7(2):e32099. doi: 10.1371/journal.pone.0032099

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Ma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: The relative level of TRX mRNA was detected by RT-PCR. β-actin was used as the reference control. The mRNA level of control was considered as 100%. The relative level of TRX mRNA was calculated using equation 2^−ΔΔCT (n = 3, mean±SD, the symbols, * and #, indicate that a value differed significantly (p<0.05) from the control and nt-TRX RNAi treated groups, respectively.); B: Reduction in TRX protein levels was confirmed by the western blot analysis in Raw264.7 cell. Lane 1 and 3 were nt-TRX RNAi plasmid treated cell lysis, lane 2 and 4 were TRX RNAi plasmid treated cell lysis. All samples were extracted at 24 h after transfection.