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. 2012 Feb 27;7(2):e31948. doi: 10.1371/journal.pone.0031948

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The binding of aptamers to Recothrom was monitored in real time using BLI and the kinetic parameters obtained using the Octet data analysis software version 7.0. (A) Correlation between K_dis values obtained in the label-free assay with the apparent K_ds estimated using the ELISA based assay (r = 0.842, P<0.05). (B) Effect of heat-treatment of human thrombins on the k_dis for all 6 aptamers. Recothrom was heat-treated as described in Fig. 3 and the k_dis determined following heat treatment for 0 and 17 h. The percent of the binding at time 0 is represented. Data presented is the mean (± SD) of three independent experiments.