Skip to main content
. 2012 Feb 27;7(2):e32348. doi: 10.1371/journal.pone.0032348

Figure 3. Gene expression and molecular characterization of goldfish pax2a mRNAs.

Figure 3

(A) Expression of two pax2a mRNA variants (pax2a_tv1 and pax2a_tv2) in goldfish optic nerve and 5 dpf zebrafish embryos by RT-PCR. MWM: molecular weight marker. (B–C) Comparison of two goldfish partial-length cDNAs, pax2a_tv1 and pax2a_tv2, with their corresponding homologous in zebrafish. Alignment produced with the ClustalW program of the nucleotide sequences of pax2a of both cyprinidae species. The primers used for the molecular characterization are underlined and indicated in Table 2. The differences in the base pairs are shown with an arrow and the similarities with an asterisk. (D–E) Comparison of goldfish Pax2a_tv1/tv2 and their corresponding homologous in zebrafish. Alignment produced with the ClustalW program of the deduced amino acid sequences of Pax2a_tv1/tv2 of both cyprinidae species. The difference in the amino acids is shown with an arrow and the similarities with an asterisk. The octapeptide domain is marked in grey.