Figure 7. Double immunolabeling S100 (red)-GFAP (green), S100 (red)-GS (green) and Pax2 (red)-GS (green) with DAPI (blue) in the ONH.

(A) S100⁺ astrocytes (arrows), S100⁺/GFAP⁺ astrocyte (asterisk), S100⁺ oligodendrocytes (arrowheads) and GFAP⁺ Müller cell vitreal processes (empty arrow) in control goldfish. (B) S100⁺ astrocytes with long processes at 7 d post-injury (arrows). Square enlarged in C. (C) Detail of the S100⁺ astrocytes with long processes (arrow) in the optic disc (D) S100⁺/GS⁺ astrocytes surrounding the posterior part of the ONH (arrow) and GFAP⁺ Müller cells with disorganized vitreal processes (empty arrow) at 7 d post-injury. (E) S100⁺/GFAP⁺ astrocytes surrounding the posterior part of the ONH and in the optic disc (arrows), S100⁺/GFAP⁺ processes (arrowhead) and GFAP⁺ Müller cells with disorganized vitreal processes (empty arrow) at 21 d post-injury. (F) S100⁺/GS⁺ astrocytes surrounding the posterior part of the ONH and in the optic disc (arrows), S100⁺/GS⁺ processes (arrowhead) and GS⁺ Müller cells with disorganized vitreal processes (empty arrow) at 21 d post-injury. (G) Pax2⁻/GS⁺ astrocytes surrounding the posterior part of the ONH (arrows) and GS⁺ Müller cells vitreal processes (empty arrow) at 21 d post-injury. (H) Few S100⁺ astrocytes are surrounding the posterior part of the ONH (arrow) and there is a great amount of GFAP⁺ Müller cell vitreal processes (empty arrows) at 60 d post-injury. Scale bars: A–B, E, H: 100 µm; C–D, F–G: 50 µm. CA: central artery; INL: inner nuclear layer; IPL: inner plexiform layer; NFL: nerve fiber layer; ONL: outer nuclear layer; PE: pigmentary epithelium; PS: photoreceptor segments.