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. 2012 Feb 27;7(2):e32348. doi: 10.1371/journal.pone.0032348

Table 1. Antibodies used for immunohistochemistry assays.

Antibody Host Description Supplier Catalogue number Dilution
Cytokeratin Mouse monoclonal Mixture of monoclonal antibodies from the following clones: C-11, PCK-26, CY-90, KS- 1A3, M20, and A53-B/A2. Sigma C2562 1∶100
GFAP Mouse monoclonal Derived from the hybridoma G-A-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified GFAP from pig spinal cord. Sigma G6171 1∶400
Pax2 Rabbit polyclonal Pax2 sequence corresponding to amino acids 188–385 (Pax2A and Pax2B) Covance PRB-276P 1∶900 (fluorescence)1∶200 (electron microscopy)
PCNA Mouse monoclonal Rat PCNA made in the protein A expression vector pR1T2T Santa Cruz Biotech. Sc-56 1∶500
S100 Rabbit polyclonal S100 isolated from bovine brain. Dako Z0311 1∶1500
Zn8 Mouse monoclonal Neuronal cell surface marker (SC-1, DM-GRASP, BEN)<INPUT VALUE = \neuronal cell surface marker (SC-1, DM-GRASP, BEN) NAME = custcol_antigen TYPE = hidden>§MACROBUTTON HTMLDirect Hybridoma Bank 1∶300
ZO1 Mouse monoclonal Human recombinant ZO1 fusion protein encompassing amino acids 334–634. Invitrogen 33–9100 1∶100