Figure 2. Overexpression of PPM1A wt but not mutant suppresses colony formation and proliferation.

( A ) PC6-3 PPM1A wt or mutant cells were seeded in different Tet concentrations as indicated and grown for 14 days, fixed and stained with Giemsa dye. Cell extracts prepared 48 hours after Tet addition were assayed for PPM1A expression by western blot using anti-PPM1A antibodies. The results shown are of a representative experiment out of three. ( B ) Two clones of PC6-3 PPM1A wt cells (clone 2 and 4) were incubated with or without Tet for 0, 24, 48 and 72 hours. The cells were then assayed for viability using the XTT assay. The average of 6 different wells ± standard deviation from a representative experiment out of three was plotted. ( C ) One clone of PC6-3 cells overexpressing PPM1A mutant was treated as in ( B ). cl- clone.