Skip to main content
. 2012 Feb 27;7(2):e32451. doi: 10.1371/journal.pone.0032451

Figure 2. BCR-JAK2 renders Ba/F3 cells growth factor-independent.

Figure 2

Ba/F3 cells were transduced in three independent experiments with pLZR-IRES-EGFP (pLZR) retroviral vector alone (Ba/F3-mock) or pLZR carrying BCR-JAK2 (Ba/F3-BCR-JAK2). Ba/F3 cells transduced with TEL-JAK2 were used as positive control (Ba/F3-TEL-JAK2). (a) Microphotographs of cultured Ba/F3-mock growing with IL-3 and Ba/F3-BCR-JAK2 and Ba/F3-TEL-JAK2 cells without IL-3 (×20 magnification). (b) For proliferation assays, 3×105 cells were incubated in the presence or absence of 10 ng/ml IL-3 for 5 days. Viable cells were counted daily by trypan blue exclusion. Ba/F3-BCR-JAK2 cells proliferate either in the presence or absence of IL-3. Results are given as mean ± SD (n = 3).