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. 2012 Feb 27;7(2):e32451. doi: 10.1371/journal.pone.0032451

Table 1. Primers and probes used for PCR analysis.

PCR Gene sequence Name 5′- 3′ Annealing temperature (°C) Cycles Product size (bp) Position (nt)
Qualitative BCR-JAK2 BCR-Fw-T GCCATGGTGGACCCGGTGG 55 35 2260 594–6121
JAK2-Rv-T AAGGTCATTTCTTTCATCCAGCC 3884–39062
Quantitative BCR-JAK2 FRET-probe-FL GTCTTGCGGACGCCCACGA-FL * 1211–12291
FRET-probe-LC640 LC640-GGTGGCCTCGGACACGACAACC+ 1188–12091
BCR-B-Fw CCCCCGGAGTTTTGAGGATTG 63 45 997 1547–15671
JAK2-3-Rv GGCCACAGAAAACTTGCTCTC 3576–35962
BCR BCR-B-Fw CCCCCGGAGTTTTGAGGATTG 63 45 301 1547–15671
BCR-B-Rv ATCGTTGGGCCAGATCTGCC 1828–18471
Bcl-xL Bcl-xL Fw TCAGAGCTTTGAGCAGGTAGTG 58 45 187 726–7473 552–5734
Bcl-xL Rv TCCCGTAGAGATCCACAAAAG 935–9553 761–7814
Osm Osm Fw AGAATCAGGCGAACCTCACGG 58 45 74 217–237 5
Osm Rv GTGTGTTCAGGTTTTGGAGGC 271–2915
Gapdh Gapdh Fw AGAAGGTGGTGAAGCAGGCATC 58 45 116 820–8416
Gapdh Rv CGGCATCGAAGGTGGAAGAGTG 915–9366
H3 H3 Fw AAAGCCGCTCGCAAGAGTGCG 62 35 201 185–2057
H3 Rv ACTTGCCTCCTGCAAAGCAC 387–4067

Fw, forward; Rv, reverse; FL, fluorescein; LC640, LightCycler Red.

*,+: inverse and complementary probe sequences to human BCR;

1

human BCR (NM_004327);

2

human JAK2 (NM_ 004972);

3

human Bcl-xL (BCLXL or BCL2L1) (NM_ 1385781);

4

mouse Bcl-xL (BCL2-like 1 or Bcl2l1) (NM_ 009743) and

5

mouse Osm (oncostatin M) (NM_001013365.2) [39];

6

mouse Gapdh (glyceraldehyde-3-phosphate dehydrogenase) NM_008084.2),

7

human H3 (H3F3A) (NM_002107.3).

Primers for Bcl-xL and H3F3A are designed to hybridize with both human and mouse sequences (patient samples and Ba/F3 cells, respectively).