Table 2. Na+- and Cl−-dependence and inhibitory effect of several compounds on [14C]GAA uptake by freshly isolated rat hepatocytes.
Conditions/Inhibitors | % of control |
Na+- and Cl—dependence | |
Control | 100±12 |
Na+-free | 35.6±2.5* |
Cl—free | 40.3±1.6* |
Inhibitory effect | |
Control | 100±4 |
Guanidinoacetic acid (GAA) | 24.1±1.1* |
GABA | 17.1±1.6* |
β-Alanine | 28.5±5.5* |
β-Guanidinopropionic acid | 18.9±0.9* |
Guanidinoethansulfonic acid | 33.0±4.4* |
Nipecotic acid | 30.8±1.2* |
Creatinine | 75.3±8.9** |
Guanidinosuccinic acid | 71.2±5.1* |
Betaine | 64.4±8.7* |
L-Arginine | 74.9±11.1* |
Glycine | 54.4±11.2* |
L-Alanine | 59.6±7.8* |
L-Serine | 83.8±6.4 |
[14C]GAA (18 µM) uptake by hepetocytes was measured at 37°C for 3 min in the absence or presence (control) of Na+ and Cl− or in the absence (control) or presence of inhibitors (2 mM). Each value represents the mean±SEM (n = 3–4).
*p<0.01,
**p<0.05, significantly different from the control.