Figure 11. Hydrogen bond interaction network for WT-ANG.

The hydrogen bond interactions between contiguous amino acid residues based on a 3.2 Å cut-off has been presented here. All hydrogen bond interaction paths up to catalytic residue His114 have been compared with the hydrogen bond interaction paths of other mutants. These hydrogen bond interactions are shown in blue square boxes. Other hydrogen bond interactions are shown in grey circles. The starting residues through which these paths led to His114 are shown in pink square boxes. The bond length is given on the edge between the nodes of amino acid residues. All the hydrogen bond interaction paths are mediating through Thr44 and there is no conformational switching of His114.