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. Author manuscript; available in PMC: 2012 Feb 27.
Published in final edited form as: Biochim Biophys Acta. 2010 Feb 11;1804(6):1301–1309. doi: 10.1016/j.bbapap.2010.01.022

Table 2.

Chaperones and molybdopterin cofactor biosynthesis proteins identified to interact with DmsD by in vitro methods.

Interactor Description Technique
Ef-Tu Translation elongation factor (TufA) 1, 3, and 4
DnaK Hsp70 Homologue; Chaperone 1, 2, 3, and 4
GroEL 60 kDa Chaperonin 1, 2, 3, 4, and 5
GrpE GrpE protein; part of the DnaK/DnaJ/GrpE system 1, 3, and 4
Tig Trigger factor; interplay with DnaK/GroEL as chaperones 1, 3, and 4
MoeB Molybdopterin biosynthesis 1, 3, and 4

Techniques:

  1. Cross-linking followed by direct LC–MS/MS for detection.
  2. Ni2+-affinity chromatography followed by SDS-PAGE and MALDI-TOF MS.
  3. Co-precipitation followed by SDS-PAGE and MALDI-TOF MS.
  4. Cross-linking followed by SDS-PAGE and MALDI-TOF MS
  5. Co-purification followed by SDS-PAGE and MALDI-TOF MS.