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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Immunother. 2012 Feb;35(2):131–141. doi: 10.1097/CJI.0b013e31824300c7

Figure 2.

Figure 2

Decitabine selectively induces expression of NY-ESO-1 protein in CRC cells. (A) Western blot analysis of NY-ESO-1 expression in normal human testis, control or DAC-treated dermal fibroblasts, and control or DAC-treated CRC cell lines. Levels of β-actin were assessed to confirm equal protein loading between paired samples. Arrowhead indicates position of NY-ESO-1 band. (B) Immunofluorescence analysis of NY-ESO-1 expression (red) in control or DAC-treated A375 melanoma and SW480 CRC cells. 4′,6-diamidino-2-phenylindole (DAPI) staining (blue) for nuclear visualization was performed, and the images were merged. Magnification is the same in all 4 panels.