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. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: Nature. 2012 Feb 8;482(7385):423–427. doi: 10.1038/nature10782

Figure 2. Visualization of RecA-promoted DNA pairing with an individual optically-trapped DNA-dumbbell, imaged by epifluorescence.

Figure 2

a, Four channel flowcell with a flow-free reservoir. b, DNA-dumbbell assembly and RecA-pairing reaction: 1) trap two beads (yellow); 2) capture λ DNA molecule (green) on one bead; 3) capture free DNA end with second bead using steerable optical trap; 4) set center-to-center bead distance, and remove YOYO-1; 5) incubate DNA-dumbbell in reservoir with RecA nucleoprotein filaments (red); and 6) extend DNA to visualize products. c, Images of pairing products with 430 and 1,762 nt nucleoprotein filaments.