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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Cancer Res. 2011 Dec 20;72(4):917–927. doi: 10.1158/0008-5472.CAN-11-1620

Figure 3.

Figure 3

Combinatorial anti-LAG-3/anti-PD-1 treatment results in enhanced adaptive immune responses. Mice were inoculated on day 0 with 2×106 MC38 cells s.c. in the right flank, euthanized at day 15, and tissues analyzed by flow cytometry. (A) Tumor draining inguinal (DLN) and non-draining brachial and axillary lymph nodes (NDLN) were isolated and activated with PMA + ionomycin for 4 h in the presence of brefeldin A, then analyzed by intracellular staining and flow cytometry (gated on lymphocytes). Nonparametric one-way ANOVA with Kruskal-Wallis test (p = 0.0074) was used for part A. (B) Tumor-infiltrating lymphocytes were analyzed by intracellular staining and flow cytometry; plots represent 5-8 animals per group. Numbers are percentage cytokine-positive infiltrating lymphocytes.