Figure 5.

Reduced tumor growth in tumor-bearing Lag3^−/−Pdcd1^−/− mice. WT, Lag3^−/−, Pdcd1^−/−, and Lag3^−/−Pdcd1^−/− mice were inoculated on day 0 with 5×10⁵ B16 cells i.d. (A), 2×10⁶ MC38 cells s.c. (B), or 5×10⁶ MC38 cells s.c. (C). Tumors were measured with an electronic caliper and reported as volume (see Methods). Data are combined from 2-3 repeated experiments, 3-5 animals per animals per group. Data were analyzed using the Maximum Likelihood method to determine synergy p values: (A) 0.0253, (B) 0.94, and (C) 0.0273 for the Lag3^−/−Pdcd1^−/− mice compared to the additive effect of the two single knockouts. Animals were euthanized when tumors became large, ulcerated, and/or necrotic.