Figure 6.
Tumor-draining (right panel) and non-draining (left panel) LN T cells were isolated on day 14 post-B16 inoculation and were activated with PMA + ionomycin for 4 h in the presence of brefeldin A. After intracellular cytokine staining, CD8+IFN-γ+ (A) and CD4+IFN-γ+ (B) cells were analyzed by flow cytometry. Data are representative of 10-15 animals per group. Nonparametric one-way ANOVA with Kruskal-Wallis test (p < 0.0001) was used; symbols represent * p<0.05, ** p<0.01, and *** p<0.001 vs Lag3−/−Pdcd1−/−.