. Author manuscript; available in PMC: 2013 Mar 1.

Published in final edited form as: Bioorg Med Chem. 2012 Jan 21;20(5):1679–1689. doi: 10.1016/j.bmc.2012.01.021

Table 1.

Kinetic constants of fungal laccase with known laccase substrates and substituted phenols.

Substrate	k_cat (min⁻¹)	K_m (mM)	k_cat/K_m (min⁻¹mM⁻¹)	ε (M⁻¹cm⁻¹)^a	Wavelength (nm)
Hydroquinone	7,130	0.02	35,650	17,252 [34]	248
catechol	2,010	0.60	3,350	2,211 [34]	450
2-aminophenol	2,250	0.19	11,840	17,865 [35]	434
1,2-phenylenediamine	1,040	0.43	2,420	13,000 [36]	440
ABTS	8,360	0.09	92,890	36,000 [34]	414
Compound 1	1,100	0.07	15,710	2,590	400
Compound 2	6,310	2.20	2,870	762	410
Compound 3	1,900	0.04	47,500	3,600	440
Compound 4	8,680	0.05	173,600	1,700	500
Compound 5	5,380	0.04	134,500	876	450
Compound 6	5,760	0.07	82,290	2,280	350
Compound 7	7,870	0.11	71,550	5,180	390
Compound 8	3,780	0.08	47,250	9,840	390
Compound 34	2,930	0.07	41,860	14,480	440
Compound 35	2,550	0.17	15,000	13,760	440

The extinction coefficients (ε) are for the oxidized products. Extinction coefficients for oxidized hydroquinone, catechol, 2-aminophenol, 1,2-phenylenediamine, and ABTS have been reported previously [34–36].