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. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: Oncogene. 2011 Jul 18;31(8):1034–1044. doi: 10.1038/onc.2011.296

Figure 3. ZBTB4 and as-miR-20a suppress Sp1, Sp3 and Sp4 transcription.

Figure 3

Decreased mRNA levels in cells transfected with ZBTB4 (A) or as-miR-20a (B). MCF-7 cells were transfected with ZBTB4 (2 µg) or as-miR-20a (100 nM) (and as-CT), and after 48 hr, Sp1, Sp3 and Sp4 mRNA levels were determined as described in the Materials and Methods. As-miR-20a and ZBTB4 decreased luciferase activity in cells transfected with pSp1For4 (C) and pSp3For5 (D). MCF-7 and MDA-MB-231 cells were transfected with the different constructs (as indicated), ZBTB4 expression plasmid (0, 1 or 2 µg), and as-miR-27a (0, 100 or 150 nM) and, after 48 hr, luciferase activity determined as described in the Materials and Methods. Results (A-D) are means ± SE (triplicate) and significant (p<0.05) inhibition (**) is indicated.