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. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: Oncogene. 2011 Jul 18;31(8):1034–1044. doi: 10.1038/onc.2011.296

Figure 7. ZBTB4 and as-miR-20 inhibit breast cancer cell growth and invasion.

Figure 7

Inhibition of MCF-7 (A) and MDA-MB-231 (B) cell growth. Cells were transfected with ZBTB4 (2 µg) or as-miR-20a (150 nM), and their effects on cell growth after 48 or 96 hr and cell invasion in a Boyden Chamber assay were determined as described in the Materials and Methods. (C) Boyden chamber assay. This assay was carried out as described for cell proliferation (32 hr) and cancer cell invasion was determined using a Boyden chamber as described in the Materials and Methods. Schematic Model to explain how miR-17-92/ZBTB4/Sp axis plays important role in oncogenesis (D). Results are expressed as means ± SE (triplicate) and significant (p<0.05) inhibition by ZBTB4 or as-miR-20a is indicated (*).