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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Clin Immunol. 2011 Dec 8;142(3):320–331. doi: 10.1016/j.clim.2011.11.010

Fig. 4. Epitope modification of immunodominant C2 peptides lowers immune recall responses to original sequences.

Fig. 4

Corresponding HLA dominant peptides and their modifications were tested for their ability to lower antigenicity of protein in DR3×E16 and DR4 mice. A: DR3×E16 mice were immunized with 2191-O. B: DR3×E16 mice were immunized with 2271-O; C: DR4 mice were immunized with 2231-O. D: DR4 mice were immunized with 2310-O. 3 μg of each peptide was emulsified in CFA. Subcutaneous injection was given to mice via a hind footpad and the base of tail. Two weeks later, T cell proliferation was determined using pooled LN cells from immunized mice (n=2). Cells were primed with indicated ORG and MOD peptides at concentrations of 0, 1, 3 and 10 μg/ml. Values are means ± SEM of triplicate cultures, p < 0.05.