Table 6.
Distribution of cells derived from IPS grafts in chimeras
| Tissue | Chimera number | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 41 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 121 | ||
| Allantois | + | + | +4 | + | + | 168 | 166 | 114 | 70 | 47 | 2 | − | |
| Vessel endothelium | Dorsal aortae | 8 | 8 | 4 | 4 | 3 | − | 1 | 26 | 6 | − | 22 | 3 |
| Omphalomesenteric artery | − | − | 17 | − | 4 | − | 6 | 18 | − | − | − | 10 | |
| Vessel of confluence | 5 | 17 | 8 | 22 | 14 | 74 | 7 | 46 | − | − | − | 4 | |
| Yolk sac | 2 | − | − | − | 9 | − | − | − | − | − | − | − | |
| Hematopoietic cells | Posterior embryo2 | − | − | − | − | − | − | − | − | − | − | − | − |
| Yolk sac blood islands | − | − | − | − | − | − | − | − | − | − | − | − | |
| Other tissues of the posterior embryo | Hindgut | 20 | 3 | 5 | − | 48 | − | 8 | 4 | − | 8 | 138 | 10 |
| Posterior mesoderm3 | + | + | + | + | 119 | + | + | + | + | + | + | + | |
| Somatopleure | 171 | 146 | 112 | + | 21 | 30 | + | + | 141 | 177 | + | 100 | |
| Splanchnopleure | + | + | + | + | 53 | + | + | + | 147 | 196 | + | + | |
| Surface ectoderm | − | − | − | − | − | 2 | − | 10 | − | 1 | − | − | |
| Ventral ectodermal ridge | − | − | − | − | − | 26 | − | 39 | − | − | − | − | |
| Other extraembryonic tissues | Yolk sac xve | 8 | − | − | − | − | − | − | − | − | − | − | − |
| Yolk sac mesothelium | − | − | − | − | − | − | − | − | − | − | − | − | |
| Amnion | − | − | − | − | − | − | − | − | − | − | − | − | |
| STELLA-positive cells | Hindgut | 1 | 1 | 5 | − | 2 | − | 1 | − | − | 4 | 3 | 2 |
| Posterior mesoderm3 | 3 | 15 | 3 | − | − | − | 14 | − | − | − | − | − | |
| Surface ectoderm | − | − | − | − | − | − | 1 | − | − | − | − | − | |
| Ventral ectodermal ridge | − | − | − | − | − | 12 | − | 7 | − | − | − | − | |
| Allantois | 1 | − | 7 | − | 1 | − | − | − | − | − | − | − | |
| Staging | Initial host stage | vEHF | EHF | EHF | EHF | EHF | LHF | EHF | LHF | LHF | EHF | LHF | LHF |
| Graft stage | vEHF | EHF | EHF | EHF | EHF | LHF | EHF | LHF | EHF | EHF | LHF | LHF | |
| Final host stage | 10-s | 9-s | 9-s | 9-s | 9-s | 11-s | 9-s | 11-s | 10-s | 10-s | 11-s | 10-s | |
Gray highlight denotes statistically significant results (p<0.05)
+ denotes greater than 200 cells
1
Chimera exhibited a host-derived double omphalomesenteric artery.
2
Circulating within dorsal aortae, omphalomesenteric artery, and/or vessel of confluence.
3
Includes general loose mesenchyme and/or mesoderm associated with somatopleure.
4
Includes contribution to ventral cuboidal mesothelium (VCM) of the allantois, observed 1 out of 12 times (p=0.2678); no contribution to dorsal cuboidal mesothelium (DCM) observed within this or any other chimera.