Table 7.
Average1 contribution of graft-derived cells occurring at a statistically significant frequency in synchronous (approximate) orthotopic grafts
| Cell type | Distal allantois (sem) |
Distal ACD (sem) |
Proximal ACD (sem) |
IPS (sem) |
|
|---|---|---|---|---|---|
| Allantois (total)2 | >200 (n/a)*** | >200 (n/a)*** | >200 (n/a)*** | 142.5 (21.9)5; *** | |
| Vessel endothelium | Dorsal aortae | − | − | 21.0 (6.6)*** | 8.5 (2.7)*** |
| Omphalomesenteric artery | 34.0 (2.0)6; * | 12.3 (8.0)** | 29.7 (10.5)*** | 11.0 (2.8)*** | |
| Vessel of confluence | − | 15.5 (13.5)* | 12.3 (4.6)*** | 21.9 (7.8)*** | |
| Yolk sac | 65.7 (42.7)6; ** | 88.7 (41.2)6; ** | 16.0 (7.1)6; *** | 5.5 (3.5)6; * | |
| Hematopoietic cells | Posterior embryo3 | − | − | 3.0 (1.3)*** | − |
| Yolk sac | − | 5.0 (3.0)6; * | − | − | |
| Other tissues of the posterior embryo | Hindgut (total)2 | − | − | 18.8 (5.3)*** | 27.1 (14.6)*** |
| Posterior mesoderm (total)2, 4 | − | − | 26.1 (13.9)*** | 193.3 (6.8)5; *** | |
| Splanchopleure | − | − | − | 141.5 (18.5)5; *** | |
| Somatopleure | − | − | 10.5 (1.9)*** | 183.0 (12.6)5; *** | |
| Surface ectoderm (total)2 | − | − | − | 10.7 (5.8)** | |
| Ventral ectoderm ridge (total)2 | − | − | − | 32.5 (6.5)* | |
| Other extraembryonic tissues | Ys xve | − | − | − | − |
| Ys mesothelium | − | − | − | − | |
| Amnion | − | − | 14.8 (9.8)6; ** | − | |
| STELLA-positive cells only | Hindgut | − | − | 5.0 (1.6)*** | 2.4 (0.53)*** |
| Posterior mesoderm4 | − | − | − | 8.8 (3.3)*** | |
| Surface ectoderm | − | − | − | − | |
| Ventral ectodermal ridge | − | − | − | 9.5 (2.5)* | |
| Allantois | − | − | 15.8 (5.5)*** | 3.0 (1.4)** | |
| Total | − | − | 16.8 (4.61)*** | 8.3 (1.84)*** | |
Average for each cell type calculated from only chimeras exhibiting contribution to that cell type.
For immunostained specimens, includes STELLA-positive and -negative cells.
Circulating within dorsal aortae, omphalomesenteric artery, and/or vessel of confluence.
Includes general loose mesenchyme and/or mesoderm associated with somatopleure.
Underestimation of average graft contribution. See Materials and Methods for details.
Contribution may be an artifact of grafting technique and reflects graft potency but not fate; see Discussion.
p<0.05;
p<0.01;
p<0.001; statistical significance based on frequency of contribution, one-tailed exact binomial test.
Abbreviations: n/a, not applicable; sem, standard error of the mean; ys, yolk sac; xve, extraembryonic visceral endoderm.